Glutathione system in rat blood serum under the conditions of carrageenan-induced joint inflammation and chondroitin sulfate prophylactic injection

The goal of this work was the research chondroitin sulfate prophylactic action on glutathione system state in rat blood serum during acute joint inflammation. performed white non-linear pubertal males weighting 160-240 grams compliance to all general ethical principles animal research. All animals were divided four experimental groups. first group – control: injected with 0,1 ml 0,9 % NaCl solution a right hind leg. second rats subjected daily intramuscular injections 3 mg/kg theurapeutic dose 28 days. third doses extremities and starting from 29th day inflammation modelled (the 1% сarrageenan injection extremities. fourth receiving therapeutic for days, after that day. 40 taking part peroxidase activity assessed judging decrease GSH amount reaction Ellman reagent. estimated by probe optical density owing NADPH oxidation. Glutathione transferase speed conjugate formation between 1-chloro-2,4-dinitrobemzene. reduced spectrophotometrically using orthophthalic aldehyde. It established carrageenan-induced peroxidase, reductase reduced, well as glutathione, whereas increased comparison control. shown under based preparate aforementioned parameters partially stabilized.